NGS technology

 NGS technology

This video was a tough one to understand. The only parts I really got were cluster generation and sequencing. For the bit on cluster generation I didn’t understand what she was talking about but I could see through the pictures/animation that clonal amplification was taking place, but I am not sure what was happening on the scientific level. The only part about sequencing I understood was that the fluorescent bases were competing to match with the original strand. The part of the video that confused me the most was sample prep and how the bridge is formed. I was also struggling with forward and reverse and why the strands were in the order that they were and what the significance of that was.